KMID : 1123920040180051418
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Korean Journal of Oriental Physiology and Pathology 2004 Volume.18 No. 5 p.1418 ~ p.1425
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Chongmyung-tang Inhibits the Cytotoxicity of Beta-amyloid in Neuro 2A Neuroblastoma Cells
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Gug Yun-Jai
Choi Hyuk Kim Tae-Heon Kang Hyung-Won Lyu Yeoung-Su
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Abstract
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The water extract of Chongmyung-tang has been traditionally used for treatment of memory-disorder in oriental medicine. This study was designed to investigate the protective mechanisms of Chongmyung-tang on ¥â-amyloid or H©üO©ü-induced cytotoxicity in Neuro 2A cells. The water extract of Chongmyung-tang significantly reduced both ¥â-amyloid or H©üO©ü-induced cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Also, it inhibited the mitochondrial dysfunction including the disruption of mitochondria membrane permeability transition(MPT) and the modulation in expression of Bcl-2 family proteins in H©üO©ü-treated H9c2 cells. Furthermore, pretreatment of quercetin inhibited the activation of caspase-3, in turn, degradation of ICAD/DFF45 were completely abolished in H©üO©ü-treated cells. Taken together, that data suggest that the protective effects of the water extract of Chongmyung-tang against ¥â-amyloid induced oxidative injuries may be achieved through modulation of mitochondrial dysfunction.
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KEYWORD
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Chongmyung-tang(ôéÙ¥÷·), ¥â-amyloid, H©üO©ü, MPT, Apoptosis, Bcl-2, ICAD/DEF45
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